Quant 3D Q150 Uživatelská příručka Strana 3
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- Tabulka s obsahem
- ŘEŠENÍ PROBLÉMŮ
- KNIHY
Hodnocené. / 5. Na základě hodnocení zákazníků
Contents
About this guide ............................................................ 8
Revision history ................................................................. 8
Purpose ....................................................................... 8
Prerequisites ................................................................... 9
Instrument hardware ............................................................ 9
Instrument software ............................................................. 9
System requirements ...................................................... 10
Instrument consumables ....................................................... 10
About the Digital PCR 20K Chip .............................................. 10
■
CHAPTER 1 Getting Started ............................................ 11
About the QuantStudio
®
3D Digital PCR System .................................... 11
Data collection ............................................................ 12
Instrument filters and supported dyes ........................................ 12
Operating the instrument ....................................................... 13
Compatible reagent kits and assays .......................................... 13
Operational workflow ...................................................... 13
Prepare the QuantStudio
®
3D Digital PCR System for use ........................... 14
About the installation ...................................................... 14
Materials required ......................................................... 14
Before you begin .......................................................... 14
Plan the laboratory layout .................................................. 15
Choose additional protective devices ......................................... 16
Install the QuantStudio
®
3D Digital PCR Instrument ............................ 17
Install the thermal cycler ................................................... 23
Connect the QuantStudio
®
3D Instrument to a network .............................. 34
Connect the QuantStudio
®
3D AnalysisSuite
™
Software ............................. 35
QuantStudio
®
3D Digital PCR System User Guide
3
- QuantStudio 1
- 3D Digital PCR System 1
- Contents 3
- (Optional) 5
- About this guide 8
- Prerequisites 9
- Instrument hardware 9
- Instrument software 9
- Instrument consumables 10
- Getting Started 11
- Data collection 12
- Instrument filters 12
- Operating the instrument 13
- Prepare the QuantStudio 14
- Plan the 15
- Choose additional 16
- Install the 17
- Digital PCR 17
- Instrument 17
- IMPORTANT! The QuantStudio 20
- The QuantStudio 21
- Install the thermal 23
- IMPORTANT! The ProFlex 24
- PCR System 26
- CAUTION HOT 27
- Install the GeneAmp 29
- PCR System 9700 29
- IMPORTANT! The GeneAmp 30
- IMPORTANT! If the GeneAmp 31
- Connect the QuantStudio 34
- 3D Instrument to a network 34
- 3D AnalysisSuite 35
- Software 35
- Prepare Samples and Load 36
- Reactions 36
- Sample dilution 37
- Determine the 37
- Prepare the 39
- X0300036 41
- Load and seal the 43
- IMPORTANT! Press the 45
- Load chips manually 50
- Digital PCR 20K 51
- IMPORTANT! Allow the 53
- Perform the PCR 58
- 08:00 AM 4/25/13 25.0°C 60
- GeneAmp® PCR System 9700 60
- Run UserUtilEditCreate 60
- Version: 3.12 60
- Materials 61
- Guidelines for 62
- Unload the 64
- Analyze the Prepared Chips 66
- Analysis workflow 67
- About the 68
- Analyze the chips 69
- Analyzing the data 73
- About the results 74
- About data quality 75
- About the data 75
- Transfer the 76
- Using the QuantStudio 77
- Troubleshooting 79
- (QuantStudio 82
- IMPORTANT! Condensation 83
- Maintenance 86
- Clean the chip tray 87
- Returning your QuantStudio 89
- 3D Instrument for service 89
- Replace the instrument fuses 90
- Replace the fuses 91
- Update the 92
- Calibrate the touchscreen 93
- View the instrument log 93
- Networking 95
- Network setup 96
- Required network 97
- Connect the 98
- Instrument to a 98
- Create a network 103
- Parts and Materials 106
- Specifications and Layout 109
- Instrument clearances 110
- Electrical specifications 111
- Environmental requirements 112
- Network requirements 113
- Symbols on this instrument 114
- Conformity 115
- Location of safety 116
- Fisher Scientific 117
- Instrument safety 117
- 3D Digital PCR Chip Loader 118
- Chemical safety 119
- Biological hazard safety 120
- Documentation and support 121
- 4 February 2015 122
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