Quant 3D Q150 Uživatelská příručka Strana 66
- Strana / 122
- Tabulka s obsahem
- ŘEŠENÍ PROBLÉMŮ
- KNIHY
Hodnocené. / 5. Na základě hodnocení zákazníků
Analyze the Prepared Chips
■
Using the QuantStudio
®
3D Digital PCR Instrument ...................... 66
■
Analyze the chips .................................................... 69
■
Analyzing the data ................................................... 73
■
Using the QuantStudio
®
3D AnalysisSuite
™
Software .................... 77
Using the QuantStudio
®
3D Digital PCR Instrument
The QuantStudio
®
3D Digital PCR Instrument performs the image capture and
subsequent preliminary analysis of QuantStudio
®
3D Digital PCR 20K Chips
following thermal cycling. The QuantStudio
®
3D Instrument processes Digital PCR
20K Chips individually, where each read lasts approximately one minute. After
reading a chip, the instrument performs an initial evaluation of the imaging data
collected during the run and displays the results on the instrument touchscreen.
QuantStudio
®
3D System data flow
For each imaged Digital PCR 20K Chip, the QuantStudio
®
3D Instrument generates
and saves a single experiment (.eds) file that contains the processed imaging data and
the results from the preliminary analysis on the instrument. Depending on the chosen
data destination, the instrument transfers the saved imaging data directly to the Life
Technologies
™
cloud for immediate access by the AnalysisSuite
™
Software, or to a
network file server or local USB drive for later manual import.
QuantStudio™ 3D
AnalysisSuite™ Software
4
About imaging and
primary analysis
66
QuantStudio
®
3D Digital PCR System User Guide
- QuantStudio 1
- 3D Digital PCR System 1
- Contents 3
- (Optional) 5
- About this guide 8
- Prerequisites 9
- Instrument hardware 9
- Instrument software 9
- Instrument consumables 10
- Getting Started 11
- Data collection 12
- Instrument filters 12
- Operating the instrument 13
- Prepare the QuantStudio 14
- Plan the 15
- Choose additional 16
- Install the 17
- Digital PCR 17
- Instrument 17
- IMPORTANT! The QuantStudio 20
- The QuantStudio 21
- Install the thermal 23
- IMPORTANT! The ProFlex 24
- PCR System 26
- CAUTION HOT 27
- Install the GeneAmp 29
- PCR System 9700 29
- IMPORTANT! The GeneAmp 30
- IMPORTANT! If the GeneAmp 31
- Connect the QuantStudio 34
- 3D Instrument to a network 34
- 3D AnalysisSuite 35
- Software 35
- Prepare Samples and Load 36
- Reactions 36
- Sample dilution 37
- Determine the 37
- Prepare the 39
- X0300036 41
- Load and seal the 43
- IMPORTANT! Press the 45
- Load chips manually 50
- Digital PCR 20K 51
- IMPORTANT! Allow the 53
- Perform the PCR 58
- 08:00 AM 4/25/13 25.0°C 60
- GeneAmp® PCR System 9700 60
- Run UserUtilEditCreate 60
- Version: 3.12 60
- Materials 61
- Guidelines for 62
- Unload the 64
- Analyze the Prepared Chips 66
- Analysis workflow 67
- About the 68
- Analyze the chips 69
- Analyzing the data 73
- About the results 74
- About data quality 75
- About the data 75
- Transfer the 76
- Using the QuantStudio 77
- Troubleshooting 79
- (QuantStudio 82
- IMPORTANT! Condensation 83
- Maintenance 86
- Clean the chip tray 87
- Returning your QuantStudio 89
- 3D Instrument for service 89
- Replace the instrument fuses 90
- Replace the fuses 91
- Update the 92
- Calibrate the touchscreen 93
- View the instrument log 93
- Networking 95
- Network setup 96
- Required network 97
- Connect the 98
- Instrument to a 98
- Create a network 103
- Parts and Materials 106
- Specifications and Layout 109
- Instrument clearances 110
- Electrical specifications 111
- Environmental requirements 112
- Network requirements 113
- Symbols on this instrument 114
- Conformity 115
- Location of safety 116
- Fisher Scientific 117
- Instrument safety 117
- 3D Digital PCR Chip Loader 118
- Chemical safety 119
- Biological hazard safety 120
- Documentation and support 121
- 4 February 2015 122
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