Quant 3D Q150 Uživatelská příručka Strana 83
- Strana / 122
- Tabulka s obsahem
- ŘEŠENÍ PROBLÉMŮ
- KNIHY
Hodnocené. / 5. Na základě hodnocení zákazníků
Observation Possible Cause Action
Condensation may have been
present on the Digital PCR 20K
Chip during imaging.
IMPORTANT! Condensation
may not be visible on the Digital
PCR 20K Chip.
• If condensation is
not
visible
– If possible, read the Digital
PCR 20K Chip again after it
has warmed to room
temperature. Once
equilibrated, the data quality,
flag, and separation of
amplified versus non-
amplified peaks should
improve noticeably.
• If condensation is visible –
Using a low-lint wipe,
remove any condensation or
Immersion Fluid from the
surface of the chip by wiping
in one direction. If
necessary, use a low-lint
wipe sprayed with
isopropanol to remove any
dried residue.
The Sample Loading Blade was
not seated correctly within the
loader arm.
Before pressing the loading
button, confirm that the Sample
Loading Blade is firmly seated
and flush against the loader arm.
Chapter 5 Troubleshooting
Troubleshooting chip images using the Chip View
5
QuantStudio
®
3D Digital PCR System User Guide
83
- QuantStudio 1
- 3D Digital PCR System 1
- Contents 3
- (Optional) 5
- About this guide 8
- Prerequisites 9
- Instrument hardware 9
- Instrument software 9
- Instrument consumables 10
- Getting Started 11
- Data collection 12
- Instrument filters 12
- Operating the instrument 13
- Prepare the QuantStudio 14
- Plan the 15
- Choose additional 16
- Install the 17
- Digital PCR 17
- Instrument 17
- IMPORTANT! The QuantStudio 20
- The QuantStudio 21
- Install the thermal 23
- IMPORTANT! The ProFlex 24
- PCR System 26
- CAUTION HOT 27
- Install the GeneAmp 29
- PCR System 9700 29
- IMPORTANT! The GeneAmp 30
- IMPORTANT! If the GeneAmp 31
- Connect the QuantStudio 34
- 3D Instrument to a network 34
- 3D AnalysisSuite 35
- Software 35
- Prepare Samples and Load 36
- Reactions 36
- Sample dilution 37
- Determine the 37
- Prepare the 39
- X0300036 41
- Load and seal the 43
- IMPORTANT! Press the 45
- Load chips manually 50
- Digital PCR 20K 51
- IMPORTANT! Allow the 53
- Perform the PCR 58
- 08:00 AM 4/25/13 25.0°C 60
- GeneAmp® PCR System 9700 60
- Run UserUtilEditCreate 60
- Version: 3.12 60
- Materials 61
- Guidelines for 62
- Unload the 64
- Analyze the Prepared Chips 66
- Analysis workflow 67
- About the 68
- Analyze the chips 69
- Analyzing the data 73
- About the results 74
- About data quality 75
- About the data 75
- Transfer the 76
- Using the QuantStudio 77
- Troubleshooting 79
- (QuantStudio 82
- IMPORTANT! Condensation 83
- Maintenance 86
- Clean the chip tray 87
- Returning your QuantStudio 89
- 3D Instrument for service 89
- Replace the instrument fuses 90
- Replace the fuses 91
- Update the 92
- Calibrate the touchscreen 93
- View the instrument log 93
- Networking 95
- Network setup 96
- Required network 97
- Connect the 98
- Instrument to a 98
- Create a network 103
- Parts and Materials 106
- Specifications and Layout 109
- Instrument clearances 110
- Electrical specifications 111
- Environmental requirements 112
- Network requirements 113
- Symbols on this instrument 114
- Conformity 115
- Location of safety 116
- Fisher Scientific 117
- Instrument safety 117
- 3D Digital PCR Chip Loader 118
- Chemical safety 119
- Biological hazard safety 120
- Documentation and support 121
- 4 February 2015 122
Supermicro 188411 manuals
Owner’s manuals and user’s guides for Server barebones Supermicro 188411.
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